Mesh Microfluidic Mixing Chamber

摘要

This document provides devices, systems, and methods for detecting pathogens in a biological sample. In some cases, the devices, systems, and methods include a microfluidic device, which includes a biological sample inlet in fluid communication with at least a first microfluidic chamber. The first microfluidic chamber holds a fibrous matrix. The fibrous matrix can carrying anticoagulant and target capture agents within the fibrous matrix. In some cases, the fibrous matrix can be a nonwoven sheet, which can entangle and retain particles bonded to the target capture agents. When in use, a biological sample is introduced into a microfluidic device to flow through the fibrous matrix to mix with the anticoagulant and contact target capture agents. The target capture agents can either remain in the fibrous matrix or flow to a target capture agent chamber. A lysis agent can be delivered through microfluidic chambers including the target capture agent and any captured targets to lyse the target and produce lysate, which can be analyzed to detect a presence of the target in the biological sample.

主权项

1. A target detection device for analyzing a biological sample comprising a microfluidic device, the microfluidic device comprising a biological sample inlet in fluid communication with at least a first microfluidic chamber, the at least a first microfluidic chamber containing a fibrous matrix, the fibrous matrix carrying anticoagulant, target capture agents, or a combination thereof within the fibrous matrix.