RECOMBINANT PHAGE AND METHODS

摘要

This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making aa plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

主权项

1. A method of making a cloned phage genome, comprising:
providing a vector; inserting a starting phage genome into the vector to provide a recombinant vector; andpropagating the recombinant vector in a vector host cell that is not a phage host cell to thereby provide the cloned phage genome.