主权项 |
1. A method for detecting JC Virus quasi species in body fluid of a human being by:
a) obtaining samples of body fluid such as urine, CSF or blood, b) extracting DNA from said body fluid, c) performing a nucleic acid amplification step on said DNA, d) selecting the JC viral DNA containing samples, e) performing a further nucleic acid amplification step on said selected JC viral DNA using a primer set comprising JC viral non-coding control region (NCCR) specific primers in order to obtain amplified JC viral non-coding control region (NCCR) DNA, f) pooling equimolar amounts of said JC viral NCCR DNA and sequencing thereafter said pooled JC viral NCCR DNA via a deep-sequencing technique, g) aligning the obtained sequences with a reference sequence such as the archetype NCCR sequence and h) detecting JC virus quasi species in said body fluid. |