| 发明名称 |
METHOD FOR DISCRIMINATING PRESENCE OR ABSENCE OF MUTATION IN DNA |
| 摘要 |
A method for discriminating the presence or absence of a mutation in a DNA having a plurality of mutated forms includes performing a thermal cycle for amplifying the DNA in the presence of a pair of primers which can bind to the DNA, a first probe which does not bind to the DNA having a mutation, can bind to the DNA having no mutation, and is labeled with a first fluorescent substance, and a second probe which can bind to both of the DNA having a mutation and the DNA having no mutation and is labeled with a second fluorescent substance which emits a light having a fluorescence wavelength different from that of a light emitted from the first fluorescent substance. |
| 申请公布号 |
US2016122807(A1) |
申请公布日期 |
2016.05.05 |
| 申请号 |
US201514923810 |
申请日期 |
2015.10.27 |
| 申请人 |
Seiko Epson Corporation |
发明人 |
UEHARA Masayuki;IDEGAMI Kotaro |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for discriminating the presence or absence of a mutation in a DNA having a plurality of mutated forms, wherein the method comprises performing a thermal cycle for amplifying the DNA in the presence of
one pair of primers which can bind to the DNA, a first probe which does not bind to the DNA having a mutation, can bind to the DNA having no mutation, and is labeled with a first fluorescent substance, and a second probe which can bind to both of the DNA having a mutation and the DNA having no mutation and is labeled with a second fluorescent substance which emits a light having a fluorescence wavelength different from that of a light emitted from the first fluorescent substance. |
| 地址 |
Tokyo JP |
