| 摘要 |
Some pairs of primers are provided, being used for detection of carbapenemase genes and carbapenemase-producing bacterial strains, and a diagnosis method using said set of primers. Furthermore, there is also disclosed, preferably, the use of probes within each amplification reaction, more preferably multiplex PCR, for selective identification and detection of carbapenemase genes from a biological sample. The primers object of this invention may be used for preparing a kit for carbapenemase genes identification and detection, preferably carbapenemases contained in bacterial strains producing said enzymes. |
| 主权项 |
1. A pair of primers selected from the group consisting of: SEQ ID No: 67-SEQ ID No: 68, SEQ ID No: 1-SEQ ID No: 2, SEQ ID No: 4-SEQ ID No: 5, SEQ ID No: 7-SEQ ID No: 8, SEQ ID No: 10-SEQ ID No: 11, SEQ ID No: 13-SEQ ID No: 14, SEQ ID No: 16-SEQ ID No: 17, SEQ ID No: 19-SEQ ID No: 20, SEQ ID No: 22-SEQ ID No: 23, SEQ ID No: 25-SEQ ID No: 26, SEQ ID No: 28-SEQ ID No: 29, SEQ ID No: 31-SEQ ID No: 32, SEQ ID No: 34-SEQ ID No: 35, SEQ ID No: 37-SEQ ID No: 38, SEQ ID No: 40-SEQ ID No: 41, SEQ ID No: 43-SEQ ID No: 44, SEQ ID No: 46-SEQ ID No: 47, SEQ ID No: 49-SEQ ID No: 50, SEQ ID No: 52-SEQ ID No: 53, SEQ ID No: 55-SEQ ID No: 56, SEQ ID No: 58-SEQ ID No: 59, SEQ ID No: 61-SEQ ID No: 62, SEQ ID No: 64-SEQ ID No: 65, SEQ ID No: 72-SEQ ID No: 73 and SEQ ID No: 75-SEQ ID No: 76. |
