DRUG SELECTION FOR BREAST CANCER THERAPY USING ANTIBODY-BASED ARRAYS

摘要

The present invention provides compositions and methods for detecting the activation states of components of signal transduction pathways in tumor cells. Information on the activation states of components of signal transduction pathways derived from use of the invention can be used for cancer diagnosis, prognosis, and in the design of cancer treatments.

主权项

1. A method for detecting the presence of a truncated receptor, said method comprising:
(a) incubating a cellular extract with a plurality of beads specific for an extracellular domain (ECD) binding region of a full-length receptor and incubating said cellular extract with a plurality of capture antibodies, wherein said plurality of capture antibodies is specific for an intracellular domain (ICD) binding region of said truncated receptor, and wherein said plurality of capture antibodies is restrained on a solid support in an array to form a plurality of captured truncated receptors; (b) incubating the plurality of captured truncated receptors with detection antibodies specific for the corresponding truncated receptors to form a plurality of detectable captured truncated receptors; (c) incubating the plurality of detectable captured truncated receptors with first and second members of a signal amplification pair to generate an amplified signal; and (d) detecting an amplified signal generated from the first and second members of the signal amplification pair.