COLOR CALIBRATION FOR PATHOLOGY SLIDES

摘要

A method and system for color calibration for digital microscopy includes obtaining an image of a stained sample and obtaining a desired stain type and density information. A synthesizing spectral absorption is determined based on the desired stain type and density, and a digital image is produced from the image of the stained sample based on a spectral absorption of the desired stain type, density, and a light source. The image is then outputted to a storage medium or display. The method also includes estimating a stain type used in the image of the stained sample, and estimating a density of the stained sample.

主权项

1. A method for color calibration for digital microscopy, comprising:
obtaining an image of a stained sample; obtaining a desired stain type; synthesizing a spectral absorption based on the desired stain type; producing a digital image from the image of the stained sample based on the spectral absorption of the desired stain type and a light source; and outputting the digital image, wherein the image of the stained sample is obtained using light sources that include one or more narrow band spectral characteristics.