| 主权项 |
1. A method for generating an oligonucleotide having a predefined sequence, the method comprising:
a. designing at least one plurality of oligonucleotides, each oligonucleotide of each plurality of oligonucleotides having a different length, each oligonucleotide having an identical predefined internal sequence, a 3′ flanking sequence at the 3′ end of the internal sequence and a 5′ flanking sequence at the 5′ end of the internal sequence, the 3′ flanking sequence comprising a primer recognition site and a restriction enzyme recognition site and the 5′ flanking sequence comprising a primer recognition site, a restriction enzyme recognition site and an additional padding nucleotide sequence, wherein each padding nucleotide sequence has a different length; b. synthesizing the at least one plurality of oligonucleotides on a solid support; and c. isolating the oligonucleotide having the correct predefined internal sequence. |
