MICROMANIPULATOR FOR A CRYOMICROTOME

摘要

A microtome system has a cryomicrotome with a sectioning device in which is provided a preparation holder and a knife edge that are guided past one another inside a working space during a sectioning operation, in order to produce thin sections of a preparation retained in the preparation holder. A micromanipulator is operable outside the working space. With the micromanipulator, a tool for retention of a specimen support is positioned proximate the knife edge during a sectioning operation in order to receive the sections that are produced, preferably for substantially stationary retention of the specimen support.

主权项

1. A method of operating a microtome system having
a cryomicrotome with a working space and a sectioning device comprised of a preparation holder and a knife edge, a micromanipulator, a tool for retaining a specimen support, and a transfer container that comprises at least one receiving opening for a said specimen support and that is replaceably insertable in the working space, wherein a portion of the micromanipulator that controls position of the tool is located outside the working space, comprising the steps of:
retaining a preparation by the preparation holder;in a sectioning operation, guiding the preparation holder and the knife edge past one another inside the working space to thereby produce thin sections of the preparation; andbefore the sectioning operation, positioning the tool by the micromanipulator to a position at which the tool retains the specimen support proximate the knife edge; andmaintaining, by the micromanipulator and the tool, the specimen support proximate the knife edge and substantially stationary during the sectioning operation and loading of a thin section of the preparation produced by the sectioning operation from the knife edge onto the specimen support.