发明名称 |
METHOD FOR EXAMINING MICROORGANISMS |
摘要 |
A method for examining microorganisms has a sampling preparation step including a fluorescence staining step of stirring and mixing certain amounts of a sample and a fluorescence staining reagent, a still standing step of leaving the solution after the fluorescence staining step to still stand for a certain time, and a dilution step of diluting the solution after the still standing step with a liquid that emits no fluorescence. |
申请公布号 |
US2016122796(A1) |
申请公布日期 |
2016.05.05 |
申请号 |
US201414894492 |
申请日期 |
2014.05.16 |
申请人 |
SATAKE CORPORATION |
发明人 |
Hosaka Yukio;Fushida Shinya;Nakata Akiko;Koike Kazuhiko |
分类号 |
C12Q1/06;G01N21/64;G01N1/30 |
主分类号 |
C12Q1/06 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for examining microorganisms to measure an amount of microorganisms in a sample, the measurement of an amount of microorganisms in a sample comprising a sampling preparation step of stirring and mixing a sample and a fluorescence staining reagent to prepare a sampling, and a calculation step of an amount of microorganisms, which is a step of calculating an amount of microorganisms from the number of light emissions in fluorescence emission obtained by irradiation of the sampling with excitation light of a specific wavelength, wherein
the sampling preparation step comprises a fluorescence staining step of stirring and mixing certain amounts of the sample and the fluorescence staining reagent, a still standing step of leaving a solution after the fluorescence staining step to still stand for a certain time, and a dilution step of diluting a solution after the still standing step with a liquid that emits no fluorescence. |
地址 |
Tokyo JP |