| 主权项 |
1. A method for reducing protein productivity in seeds, comprising expressing, in a plant, a chimeric protein obtained by fusing a transcription factor and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor,
wherein said transcription factor is a protein comprising the amino acid sequence of SEQ ID NO: 2, or a protein having transactivation activity and comprising the amino acid sequence of SEQ ID NO: 2 but in which 1-10 amino acids have been deleted, substituted, added, or inserted, and wherein the functional peptide has an amino acid sequence expressed by any one of the following formulae (1) to (8):
X1-Leu-Asp-Leu-X2-Leu-X3 (1) (SEQ ID NO: 392 with deletion of 0-10 residues from the N-terminus) (where X1 denotes a set of 0 to 10 amino acid residues, X2 denotes Asn or Glu, and X3 denotes a set of at least 6 amino acid residues);
Y1-Phe-Asp-Leu-Asn-Y2-Y3 (2) (SEQ ID NO: 393 with deletion of 0-10 residues from the N-terminus (where Y1 denotes a set of 0 to 10 amino acid residues, Y2 denotes Phe or Ile, and Y3 denotes a set of at least 6 amino acid residues);
Z1-Asp-Leu-Z2-Leu-Arg-Leu-Z3 (3) (SEQ ID NO: 394 with deletion of 0-10 residues from the C-terminus and deletion of 0-2 residues from the N-terminus) (where Z1 denotes Leu, Asp-Leu, or Leu-Asp-Leu, Z2 denotes Glu, Gln, or Asp, and Z3 denotes a set of 0 to 10 amino acid residues);
Asp-Leu-Z4-Leu-Arg-Leu (4) (where Z4 denotes Glu, Gln, or Asp) (residues 4-9 of SEQ ID NO.: 394);
α1-Leu-β1-Leu-γ1-Leu (5) (SEQ ID NO: 395);
α1-Leu-β1-Leu-γ2-Leu (6) (SEQ ID NO: 396);
α1-Leu-β2-Leu-Arg-Leu (7) (SEQ ID NO: 397); and
α2-Leu-β1-Leu-Arg-Leu (8) (SEQ ID NO: 398) (where α1 denotes Asp, Asn, Glu, Gln, Thr, or Ser; α2 denotes Asn, Glu, Gln, Thr, or Ser; β1 denotes Asp, Gln, Asn, Arg, Glu, Thr, Ser, or His; β2 denotes Asn, Arg, Thr, Ser, or His; γ1 denotes Arg, Gln, Asn, Thr, Ser, His, Lys, or Asp; and γ2 denotes Gln, Asn, Thr, Ser, His, Lys, or Asp, in formulae (5) to (8)). |
