ASSAYS

摘要

Abstract A method, comprising (a) providing an amount of a reporter compound; a first binding member being configured to bind an anchor group of a capture molecule; a second binding member capable of capturing the reporter compound; an amount of a 5 target nucleic acid capable of forming complexes with the reporter compound, the forming of complexes with the reporter compound inhibiting capturing of the reporter compound by the second binding member; and an amount of capture molecules wherein each capture molecule comprises a binding portion specific to a region of the target nucleic acid and an anchor group; (b) forming complexes each 10 comprising a target nucleic acid and a capture molecule; (c) contacting the complexes with the first binding member to bind at least a subset of the complexes to the first binding member; (d) releasing at least a subset of the amount of target nucleic acid from the first binding member; (e) forming complexes of a subset of the amount of reporter compound with at least a subset of the amount of target nucleic 15 acid; (f) capturing a remaining subset of the amount of reporter compound not in complex with a target nucleic acid on the second binding member; and (g) determining a value indicative of the presence and/or amount of reporter compound captured on the second binding member.