METHODS AND SYSTEMS FOR ANALYSIS OF FIBERS AND BRANCHING STRUCTURES WITHIN AN IMAGE OF A SAMPLE

摘要

A computer-implemented method and system of fiber analysis and reconstruction of a fiber-containing biological sample perform efficient and accurate analysis of fiber morphology. The images of objects comprising fiber and/or branching structure are obtained by camera and/or other imaging devices. Variable modules are operable to obtain initial tracing seeds via adaptive threshold method for identifying tracing seeds candidates by local Principal Component Analysis (PCA) calculation and sorting out the seeds with low and high score. Each single fiber segment of interest is traced repeatedly utilizing PCA fitting calculation, and individual fiber segments are assembled by performing crossover matching calculation.