Insect resistant cotton plants and methods for identifying same

摘要

The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

主权项

1. A method for identifying elite event EE-GH6 in biological samples, which method comprises amplifying a nucleic acid present in said biological samples with at least two primers, wherein one of said primers recognizes the 5′ or 3′ flanking region of EE-GH6 and the other primer recognizes the foreign DNA of EE-GH6 contiguous therewith, said 5′ flanking region having the nucleotide sequence of SEQ ID No. 1 from nucleotide 1 to nucleotide 140 or the nucleotide sequence of SEQ ID No. 11 from position 1 to position 463 and said foreign DNA contiguous with said 5′ flanking region comprises the complement of SEQ ID No. 1 from nucleotide 141 to nucleotide 192, said 3′ flanking region having the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 113 to nucleotide 438 and said foreign DNA contiguous with said 3′ flanking region comprises the nucleotide sequence of SEQ ID No. 2 from nucleotide 1 to nucleotide 112.