Method for quantifying human DNA using an internal control

摘要

The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.

主权项

1. A method of quantifying and/or detecting one or more nucleic acids of a genome in a sample, the method comprising:
a. amplifying, in a reaction well or a reaction vial, a nucleic acid comprising a non-repetitive, multicopy locus, within the genome, the multicopy locus having copies on at least 2 chromosomes and a sequence that shares at least 80% sequence identity over a stretch of 80 base pairs to a nucleic acid sequence of SEQ ID NO:1 to produce a first amplification product; b. amplifying, in parallel in the reaction well or the reaction vial, an internal control nucleic acid sequence to produce a second amplification product; c. detecting the first and the second amplification products; and d. quantifying the first and the second amplification products; wherein amplifying the internal control nucleic acid sequence comprises amplifying with at least one primer sequence selected from the group consisting of SEQ ID NO:13 and SEQ ID NO:14.