主权项 |
1. A centrifugal separation kit installed to a centrifugal separator to divide blood into blood cell components composed of a Red Blood Cell (RBC) layer and blood plasma components composed of a Platelet Rich Plasma (PRP) layer and a PPP (Platelet Poor Plasma) layer in order from a bottom thereof, comprising:
a) an injector having an opening at an upper portion thereof so that a piston having a rubber packing installed thereto is movably inserted into the upper portion through the opening, the injector having a first predetermined volumetric capacity corresponding to a known volume of whole blood (Wb) and an insert portion at a lower portion thereof to which an injector needle is installed thereto; and b) a centrifugal separation tube loadable on the centrifugal separator with the injector installed thereto, comprising:
a lower portion having a second predetermined volumetric capacity,a collecting unit having a collecting channel of an elongated shape at a center thereof, wherein the lower portion of the centrifugal separation tube is connected with a lower end of the collecting channel and the insert portion of the injector is frictionally engaged into an upper end of the collecting channel, thereby the collecting channel providing a direct fluid communication between the lower portion and an internal space of the injector while the blood is being centrifugally separated, wherein the centrifugal separation tube is configured for including a predetermined amount of cell isolation gel having a volume of equal or greater than 0, and wherein a lower side of the collecting unit has an adjusted location so that the second predetermined volumetric capacity is substantially equal to the sum of the volume of the cell isolation gel and a predetermined fraction of the first predetermined volumetric capacity, wherein the predetermined fraction corresponds to a known volume of RBCs (Vb) present in the known volume of whole blood (Wb). |