| 发明名称 |
Engineered lower eukaryotic host strains for recombinant protein expression |
| 摘要 |
The present invention relates to novel engineered lower eukaryotic host cells for expressing heterologous proteins and to methods of generating such strains. |
| 申请公布号 |
US9328367(B2) |
申请公布日期 |
2016.05.03 |
| 申请号 |
US201214354628 |
申请日期 |
2012.10.23 |
| 申请人 |
Merck Sharp & Dohme Corp. |
发明人 |
Jiang Bo;Argyros Rebecca D.;Nelson Stephanie;Davidson Robert C.;Chen Ronghua;Zhuang Jun |
| 分类号 |
C12P21/00;C12N15/80;C07K16/32;C07K14/39 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
Fuentes Gloria;Thampoe Immac |
| 主权项 |
1. An engineered Pichia pastoris host cell that has been modified to reduce or eliminate the activity of an ATT1 gene, wherein the ATT1 gene encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:7, wherein the activity of the ATT1 gene is reduced or eliminated by: (i) reducing or eliminating the expression of an ATT1 gene or polypeptide, or (ii) expressing a mutated form of an ATT1 gene; and wherein the host cell further comprises a nucleic acid encoding a recombinant protein. |
| 地址 |
Rahway NJ US |
