Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same

摘要

Methods for producing substantially pure cultures of human neural retinal progenitor cells, forebrain progenitor cells, and retinal pigment epithelial cells are disclosed. In addition, the successful differentiation of human embryonic stem cells and human induced pluripotent stem cells through the major developmental stages of human retinogenesis is disclosed.

主权项

1. A cell culture comprising: a human vesicular neurosphere, wherein the human vesicular neurosphere includes a hollow center and an outer ring-like laminar layer comprising human retinal progenitor cells oriented radially outwards relative to the neurosphere center, wherein the outer ring-like laminar layer appears phase-bright and golden in color when observed using bright-field microscopy, wherein the human vesicular neurosphere is capable of generating cells of neural retina or retinal pigment epithelium (RPE), and wherein at least 80% of the cells within the human vesicular neurosphere are human retinal progenitor cells.