| 摘要 |
A method for isolating nucleic acid from a cerebrospinal fluid (CSF) sample, comprises adding carrier nucleic acid and protease to a CSF sample having a volume of at least 1 ml, incubating the sample comprising the carrier nucleic acid and the protease, applying the incubated sample to a nucleic acid binding column, washing the column to which the sample was applied, and applying eluent to the column resulting in the isolation of the nucleic acid. A method for determining the amount of JC virus DNA in a sample comprises performing Real-time PCR on the sample. The Real-time PCR primers and probe are directed to the JC virus T antigen, and the sequences of the Real-time PCR primers and probe are SEQ ID NOs:1-2 and SEQ ID NO:3, respectively given I the specification. |
