MEASURING DEVICE AND SYSTEM FOR PERFORMING MELTING CURVE ANALYSIS OF A DNA MICROARRAY AND UTILIZATION OF A FLUORESCENCE DETECTOR ARRAY FOR ANALYSIS

摘要

What is shown is a measuring device for simultaneously analyzing melting curves of DNA samples in DNA microarrays with the aid of a fluorescence detector array. Embodiments show monitoring of melting curves of DNA microarrays (dynamic fluorescence measurement) by means of silicon photomultipliers (SiPM) or other photodetectors such as, e.g., PIN diodes (positive intrinsic negative diodes), avalanche photodiodes (APD), or photomultiplier tubes (PMT). The DNA microarray is applied, along with a thin-film heating element, to a substrate made of plastic or glass or is integrated in a microfluidic channel.

主权项

1. A measuring device for performing melting curve analysis of at least one DNA sample, the measuring device comprising:
a two-dimensional DNA microarray for accommodating the at least one DNA sample; a two-dimensional fluorescence detector array comprising at least one fluorescence detector; and an integrated heating element for heating the DNA sample applied to the DNA microarray.