The present invention provides a production method for neural cells or nerve tissue, the method including steps (1)-(3): (1) a first step for culturing pluripotent stem cells in the absence of feeder cells in a culture medium that includes 1) a TGF-β family signal transduction pathway inhibitor and/or a sonic hedgehog signal transduction pathway activator and 2) a non-differentiation maintenance factor; (2) a second step for culturing the cells obtained in the first step in suspension so as to form an aggregate of cells; and (3) a third step for culturing the aggregate of cells obtained in the second step in suspension in the presence or absence of a differentiation inducing factor so as to obtain an aggregate that includes nerve cells or nerve tissue.
申请公布号
WO2016063985(A1)
申请公布日期
2016.04.28
申请号
WO2015JP80016
申请日期
2015.10.23
申请人
SUMITOMO DAINIPPON PHARMA CO., LTD.;RIKEN;SUMITOMO CHEMICAL COMPANY, LIMITED