Method for activating the proliferation of stem cells and increasing the resistance thereof to adverse effects

摘要

The invention relates to the field of biotechnology, medicine, pharmacology, cell biology and bioengineering and to other fields of biology and medicine in which stem cells and progenitor cells with a different level of differentiation and mature differentiated cells of different tissues are used, in particular for the accelerated production of bioimplants from donor or auto­immune stem cells in humans and/or animals. The technical result addressed by the invention consists in activating the proliferation of stem cells and increasing the resistance of human and animal stem cells to adverse effects, which is not associated with the negative action of a high-frequency electromagnetic field. The technical result is achieved with the aid of processing of a cell culture with a weak magnetic field having a low frequency, which is colinear with the permanent magnetic field of the Earth. When implementing the method, the following are observed: 1). An increase in the number of human stem cells in a culture after exposure of said stem cells to a weak magnetic field during the course of a duplication half-period which is characteristic of this stem culture, for example in 24 hours the number of cells increases by more than 2.5 times (in the case of a full period of duplication of intact cells sampled equal to 48 hours). 2). An increase in the amplitude of the intrinsic magnetic radiation of the initial culture of stem cells measured by a SQUID magnetometer, which is evidence of the increased activity of said stem cells. 3). A twofold increase in the resistance of human stem cells to the development of apoptosis and synchronization of the cells preferably in the G1 cell cycle phase.