GROUP OF GLYCOSYLTRANSFERASES AND USE THEREOF

摘要

Provided are the use of glycosyltransferases gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29-4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3, 3GT4 and derived polypeptides therefrom in the catalyzed glycosylation of terpenoid compounds and the synthesis of new saponins, wherein the glycosyltransferases can specifically and efficiently catalyze tetracyclic triterpenoid compound substrates at positions C-20 and/or C-6 and/or C-3 during hydroxyl glycosylation, and/or transfer the glycosyl from a glycosyl donor to the first glycosyl of the tetracyclic triterpenoid compounds at position C-3, so as to extend the sugar chain. The glycosyltransferases can also be used for constructing man-made synthetic rare ginsenosides and a variety of new ginsenosides and derivatives thereof.

主权项

1. A method for in vitro glycosylation, wherein said method comprises:
in the presence of a glycosyltransferase, transferring a glycosyl from a glycosyl donor to the following site on tetracyclic triterpenoid compounds: positions C-20, C-6, C-3 or the first glycosyl at position C-3; thereby forming glycosylated tetracyclic triterpenoid compounds.