METHOD OF ANALYZING BINDING ASPECT OF MEMBRANE PROTEIN IN A LIVING CELL

摘要

The present invention relates to a method for analyzing the pattern of live intercellular membrane protein binding. The method for analyzing the pattern according to the present invention can analyze accurately, sensitively, quickly, and readily the binding pattern of a target membrane protein and a candidate substance to be specifically bound therewith without tagging to a ligand, and thus measure directly and accurately the position and quantitative information of the binding of the membrane protein and the target substance. Such effects make it possible to apply the method for various uses such as dissociation constant, mutant study, complex formation, and signal transduction. Moreover, it is expected to use the method for searching out undiscovered membrane proteins and target substances.

主权项

1. A method of analyzing a binding aspect between a candidate material and a target membrane protein in a living cell, the method comprising:
obtaining diffusion coefficients of a target membrane protein before and after treatment with a candidate material in a living cell expressing the target membrane protein, and analyzing a change in diffusion coefficient of at least one target membrane protein obtained thereby.