PROTEOLYTIC RELEASE OF CELL SURFACE ANTIGENS FOR PHAGE BIOPANNING

摘要

The invention described herein features methods of isolating monoclonal antibodies or polypeptides that bind to a cell surface expressed antigen. The method of catch and release utilizes engineered protease site for cleavage antigen-antibody or antigen-polypeptide complexes. In some embodiments the protease cleavage site to cleave the complexes is an exogenous protease to mammalian cell. In various embodiments the protease cleavage site to cleave the complexes is an endogenous protease to mammalian cell.

主权项

1. A method of selecting an antibody that binds to a protein of interest, the method comprising the steps of:
a) providing a composition comprising a mammalian cell expressing a protein of interest on the cell surface, wherein the protein of interest comprises at least one unique protease cleavage site; b) exposing the mammalian cell to a library of phage-antibody constructs; c) binding one or more phage-antibody constructs from the library to the protein of interest to form one or more antigen-antibody complexes, wherein the antigen is the protein of interest; d) releasing the one or more antigen-antibody complexes from the cell by cleaving the protease cleavage site with a protease; e) propagating the released antigen-antibody complexes and identifying the antibody that binds to the protein of interest; thereby selecting the antibody.