| 摘要 |
A fast wide field-of-view volume holographic fluorescence micro-imaging system, including a laser light source (1), a dichroic spectroscope (2), a micro-objective (3), an MEMS micro-mirror array device (4), a volume holographic grating device (5), an imaging lens (6) and an image detector array (7). The laser light source (1) provides intensity-uniform illumination conditions for an imaging target (8) and emits a lighting optical wave to the dichroic spectroscope (2). The dichroic spectroscope (2) emits the lighting optical wave to the imaging target (8). The fluorescent light emitted by the imaging target (8) returns to the dichroic spectroscope (2). The dichroic spectroscope (2) filters the lighting optical wave and projects the fluorescent light to the MEMS micro-mirror array device (4) via the micro-objective (3). The MEMS micro-mirror array device (4) carries out angular coding on light waves at different positions according to a central wavelength of an imaging spectrum and a Bragg characteristic parameter of the volume holographic grating device (5) to further control a deflection direction of an imaging light beam, and the deflected imaging light beam is incident on the volume holographic grating device (5) at a matching angle. The volume holographic grating device (5) diffracts the incident light subjected to spatial angular coding, and diffraction light is imaged to the image detector array (7) via the imaging lens (6). |
