This disclosure provides, among other things, a composition comprising: a 5′ exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
申请公布号
EP3011024(A2)
申请公布日期
2016.04.27
申请号
EP20150763701
申请日期
2015.08.27
申请人
NEW ENGLAND BIOLABS, INC.
发明人
HSIEH, PEI-CHUNG;SUN, LUO;EVANS, THOMAS, C., JR.;DAVIS, THEODORE, B.;GARDNER, ANDREW