Method and composition for staining and processing a urine sample

摘要

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

主权项

1. A particle contrast agent composition for staining particles of a urological fluid sample for imaging in an automated particle analysis system comprising:
Crystal Violet present in amounts sufficient to result in concentrations between 50 μM and 500 μM under staining conditions; and a permeabilizing agent including a 5-part differential (5 PD) lytic reagent including saponin, wherein the saponin is present in amounts sufficient to result in concentrations of about 0.01 mg/L to about 100 mg/L under staining conditions.