Systems and methods for identifying and disrupting cellular organelles

摘要

This invention relates to optomechanical systems and methods for altering, modifying or disrupting a target object. Such systems and methods are used for, for example, ablating the endogenous nucleus in a cell.

主权项

1. A method comprising:
(a) directing a target object comprising an organelle to a platform that receives an ablation radiation (b) photosensitizing the organelle to the ablation radiation; (c) generating an image of the target object on the platform; (d) generating an outline of the photosensitized organelle based upon the image; and (e) emitting the ablation radiation toward the specimen to selectively disrupt the photosensitized organelle wherein the emitting comprises at least one of:
(i) emitting the ablation radiation based on a shape determined by the organelle outline;(ii) translocating the ablation radiation in a movement pattern determined by the organelle outline; and(iii) maintaining the ablation radiation substantially stationary while translocating the target object in a movement pattern determined by the organelle outline.