| 主权项 |
1. A process for the production of butadiene comprising:
(a) culturing by fermentation in a sufficient amount of nutrients and media a non-naturally occurring microbial organism that produces crotyl alcohol thereby biosynthetically producing crotyl alcohol, wherein said microbial organism comprises a crotyl alcohol pathway comprising at least two exogenous nucleic acids encoding crotyl alcohol pathway enzymes expressed in a sufficient amount to produce crotyl alcohol, wherein said crotyl alcohol pathway comprises converting crotonyl-CoA to crotyl alcohol using at least one exogenous nucleic acid of said two exogenous nucleic acids, wherein said crotyl alcohol pathway enzymes are selected from the group consisting of: (i) a crotonyl-CoA reductase (aldehyde forming), and a crotonaldehyde reductase (alcohol forming); (ii) a crotonyl-CoA reductase (alcohol forming); and (iii) a crotonyl-CoA hydrolase, synthetase, or transferase, a crotonate reductase, and a crotonaldehyde reductase (alcohol forming), and wherein said crotyl alcohol pathway further comprises at least one exogenous nucleic acid of said two exogenous nucleic acids that encodes a crotyl alcohol pathway enzyme for converting: (i) acetyl-CoA to crotonyl-CoA using an acetyl-CoA:acetyl-CoA acyltransferase, an acetoacetyl-CoA reductase and a 3-hydroxybutyryl-CoA dehydratase; (ii) glutaconyl-CoA to crotonyl-CoA using a glutaconyl-CoA decarboxylase; (iii) glutaryl-CoA to crotonyl-CoA using a glutaryl-CoA dehydrogenase; (iv) 3-aminobutyryl-CoA to crotonyl-CoA using an 3-aminobutyryl-CoA deaminase; or (v) 4-hydroxybutyryl-CoA to crotonyl-CoA using a 4-hydroxybutyryl-CoA dehydratase; and (b) converting the crotyl alcohol produced by culturing said non-naturally occurring microbial organism in step (a) to butadiene using a chemical procedure. |
