B-cell antigen presenting cell assay

摘要

Disclosed herein are methods for diagnosing or predicting B-cell rejection in a subject. In one example, for assessing transplant rejection, the method includes determining an antigen presenting index by comparing uptake of a donor antigen to uptake of a reference antigen in a biological sample obtained from the subject. In another example, for assessing GVHD, the method includes determining an antigen presenting index by comparing uptake of a recipient antigen to uptake of a reference antigen in a biological sample obtained from the subject.

主权项

1. A method, comprising:
obtaining antigen presenting cells (APCs); contacting a first sample of the obtained APCs with a donor antigen from a donor under conditions sufficient to induce uptake of the donor antigen; contacting a second sample of the obtained APCs with a third-party antigen under conditions sufficient to induce uptake of the third-party antigen; and measuring uptake of the donor antigen in the first sample and uptake of the third-party antigen in the second sample by flow cytometry.