Ablative immunotherapy

摘要

The disclosure herein relates generally to immunotherapy and, more specifically, to the use of immunotherapy for treating tumors and pathogen infected tissues. The immunotherapy relates to first priming patients with allogeneic cells designed to be rejected by a Th1 mediated mechanism, then inducing in situ necrosis or apoptosis in a tumor or pathogen infected lesion. Necrosis or apoptosis can be induced by methods such as cryotherapy, irreversible electroporation, chemotherapy, radiation therapy, ultrasound therapy, ethanol chemoablation, microwave thermal ablation, radiofrequency energy or a combination thereof applied against at least a portion of the tumor or pathogen infected tissue. One or more doses of allogeneic cells (e.g., Th1 cells) are then delivered within or proximate to the tumor or pathogen-infected tissue in the primed patient. The present invention provides an immunotherapeutic strategy to develop de-novo systemic (adaptive) immunity to a tumor or pathogen.

主权项

1. A method of inducing an adaptive immune response against a tumor in a patient, the method comprising: generating antigens from the tumor in situ by ablation of the tumor, inducing necrotic death of tumor cells; and administering allogeneic T-cells to the site of the in situ generated antigens thereby creating an immune response that serves as an adjuvant to the uptake of antigens from the ablated tumor whereby subsequent maturation of the patient's antigen presenting cells systemically stimulate anti-tumor immunity.