RECOMBINANT E.COLI PRODUCING UNSATURATED FATTY ACID, AND METHOD FOR PRODUCING UNSATURATED FATTY ACID USING THE SAME

摘要

The present invention relates to recombinant E. coli producing, among unsaturated fatty acids, cis-vaccenic acid; and to a method for producing unsaturated fatty acids using the same. The recombinant E. coli producing unsaturated fatty acids according to the present invention constructs a system co-expressing 5 genes, i.e., accA, accB, accC, accD, fabD, and fabD located in one plasmid; 3 genes, i.e., fabB, fabF, and fabA, located in one plasmid; and tesA located in one plasmid. Also, the four recombinant plasmids are introduced into the recombinant E. coli (BL21(DE3) ΔfadD), which is a host strain having alleviated fatty acid reduction by removing fadD in a beta-oxidation step of fatty acid synthesis, and causes simultaneous transformation leading to a co-expression of 13 genes. In addition, when fatty acids are prepared by culturing the recombinant E. coli at a low temperature, the ratio and quantity of vaccenic acid, among unsaturated fatty acids, are greatly increased, so the unsaturated fatty acids can be effectively produced.