发明名称 |
METHOD FOR MANUFACTURING CELL STRUCTURE, AND CELL STRUCTURE MANUFACTURED BY THE METHOD |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for manufacturing a cell structure in which cells are allowed to spontaneously form a cell structure without particularly using an artificial scaffold, and a cell structure having a lumen structure (tubular structure) can be provided, and a cell structure manufactured by the method.SOLUTION: A method for manufacturing a cell structure comprises: a making process of making a low cloud point type temperature-responsive polymer composition; a preparation process of preparing a partially covered cell culture sheet having a covered cell culture sheet area which is provided so as to cover a part of the culture surface of a cell culture sheet by the low cloud point type temperature-responsive polymer composition and surround a first non-covered cell culture sheet area, and provided so as to be surrounded by a second non-covered cell culture sheet; a dissemination process of disseminating cells on a culture surface of the partially covered cell culture sheet at the cell density of 1.0×10/mmor more; and a culture process of culturing disseminated cells. There is also provided a cell structure manufactured by the method.SELECTED DRAWING: Figure 1 |
申请公布号 |
JP2016059337(A) |
申请公布日期 |
2016.04.25 |
申请号 |
JP20140190481 |
申请日期 |
2014.09.18 |
申请人 |
NATIONAL CEREBRAL & CARDIOVASCULAR CENTER;BRIDGESTONE CORP |
发明人 |
NAKAYAMA YASUHIDE;IWAI RYOSUKE;NEMOTO YASUSHI |
分类号 |
C12N5/07;C12N5/071;C12N5/077 |
主分类号 |
C12N5/07 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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