| 发明名称 |
MODULAR SYNTHETIC GAL1 PROMOTER AND USE THEREOF |
| 摘要 |
The present invention relates to a nucleic acid molecule that comprises at least one synthetic GAL1 core promoter containing one or more ligand responsive operator sequences, preferably one or more FadR operator sites; at least one upstream enhancer element (UEE); and at least one nucleotide sequence encoding for one or more genes of interest; wherein the at least one synthetic GAL1 core promoter is operably linked to the at least one nucleotide sequence encoding for one or more genes of interest and the at least one UEE is operably linked to the at least one synthetic GAL1 core promoter. Also encompassed are expression systems and recombinant cells that include these nucleic acid molecules and methods that use these nucleic acid molecules, expression systems or cells. |
| 申请公布号 |
US2016108410(A1) |
申请公布日期 |
2016.04.21 |
| 申请号 |
US201414889755 |
申请日期 |
2014.01.20 |
| 申请人 |
NANYANG TECHNOLOGICAL UNIVERSITY |
发明人 |
CHANG Matthew Wook;TEO Wei Suong |
| 分类号 |
C12N15/81;C12P21/00 |
主分类号 |
C12N15/81 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. Nucleic acid molecule comprising:
(i) at least one synthetic GAL1 core promoter containing one or more ligand responsive operator sequences, preferably one or more FadR operator sites; (ii) at least one upstream enhancer element (UEE); and (iii) at least one nucleotide sequence encoding for one or more genes of interest;wherein the at least one synthetic GAL1 core promoter is operably linked to the at least one nucleotide sequence encoding for one or more genes of interest and the at least one upstream enhancer element (UEE) is operably linked to the at least one synthetic GAL1 core promoter. |
| 地址 |
Singapore SG |
