发明名称 |
BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION |
摘要 |
Resonance energy transfer (RET)- or protein-fragment complement assay (PCA)- based biosensors useful for assessing the activity of G-proteins are described. These biosensors are based on the competition between the Gα subunit and a Gβγ interacting protein (βγ IP) for the binding to the Gβγ dimer. These biosensors comprises (1) a βγ IP and (2) a Gβ or Gγ protein; a GPCR; or a plasma membrane targeting domain, fused to suitable RET or PCA tags. Methods using such biosensors for different applications, including the identification of agents that modulates G-protein activity or for the characterization of GPCR signaling/regulation, such as G-protein preferences and activation profiles of GPCRs, are also described. |
申请公布号 |
WO2016058094(A1) |
申请公布日期 |
2016.04.21 |
申请号 |
WO2015CA51032 |
申请日期 |
2015.10.14 |
申请人 |
UNIVERSITÉ DE MONTRÉAL |
发明人 |
BOUVIER, MICHEL;LE GOUILL, CHRISTIAN;HOGUE, MIREILLE;LUKASHEVA, VIKTORIYA |
分类号 |
C07K14/47;C07K19/00;C12N15/12;C12N15/62;G01N33/53 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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地址 |
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