| 发明名称 |
IMPROVED SURFACE DISPLAY OF FUNCTIONAL PROTEINS IN A BROAD RANGE OF GRAM NEGATIVE BACTERIA |
| 摘要 |
The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the trans porter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell. |
| 申请公布号 |
US2016108407(A1) |
申请公布日期 |
2016.04.21 |
| 申请号 |
US201414774973 |
申请日期 |
2014.06.04 |
| 申请人 |
AUTODISPLAY BIOTECH GMBH |
发明人 |
JOSE Joachim;TEESE Mark George;SICHWART Shanna;TOZAKIDIS Iasson |
| 分类号 |
C12N15/62;C12N9/42;C12N15/63 |
主分类号 |
C12N15/62 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for displaying a recombinant polypeptide on the surface of a host cell, said method comprising the steps:
(a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell. |
| 地址 |
Düsseldorf DE |
