Method And System For Simultaneous Decomposition Of Multiple Hyperspectral Datasets And Signal Recovery Of Unknown Fluorophores In A Biochemical System

摘要

A system and method are for analyzing fluorescence of fluorophors in an eye using a non-negative matrix factorization (NMF) method. The NMF method may be initialized with Gaussian mixture model fits and may optionally be constrained to provide identical abundance images for data obtained in response to two or more excitation wavelengths.

主权项

1. A method for analyzing fluorescence of fluorophors in an eye, comprising:
obtaining, from a plurality of hyperspectral sensors, a first data matrix representative of a first electromagnetic emission spectrum from the fluorophors in response to an excitation signal; isolating a second data matrix from the first data matrix, the second data matrix being representative of a second electromagnetic emission spectrum from a retinal pigment epithelium (RPE) of the eye in response to the excitation signal; identifying one or more Gaussian functions, wherein each Gaussian function is an initial approximation representative of a spectral component of the second electromagnetic emission spectrum; and analyzing the second data matrix using non-negative matrix factorization initialized with the one or more Gaussian functions to generate one or more component matrices, wherein each component matrix is a second approximation representative of the corresponding spectral component of the second electromagnetic emission spectrum.