NANO-LIPOSOMAL AMINOGLYCOSIDE-THYMOQUINONE FORMULATIONS

摘要

The nano-liposomal aminoglycoside-thymoquinone formulations (100) are suitable for administration to a mammal and include an aminoglycoside antibiotic (amikacin, gentamicin, or tobramycin) and thymoquinone (TQ) encapsulated within a liposome. The liposome-encapsulated aminoglycoside-thymoquinone (TQ) formulations (100) can be administered to a subject in need thereof. The liposome-encapsulated aminoglycoside- thymoquinone formulations (100) are prepared by a method, which comprises forming a lipid film from a mixture of phospholipids and cholesterol; mixing the lipid film with methanol containing thymoquinone in molar ratio; evaporating off the methanol from the mixture; adding polysaccharides and/or polyethylene glycol (PEG) in PBS (phosphate-buffered saline) buffer in volume ratio to form a liposomal mixture; sonicating the liposomal mixture for at least five minutes; adding an aminoglycoside antibiotic in molar ratio with the thymoquinone; sonicating the mixture for at least five minutes; lyophilizing the liposomal aminoglycoside- thymoquinone formulation; and storing it at -80 °C until use.