| 发明名称 |
REPROGRAMMING OF AGED ADULT STEM CELLS |
| 摘要 |
A method comprising (a) obtaining young adult stem cells (YASC) from a first subject; (b) lysing the YASC to generate a lysate comprising an intracellular matrix (ICM) and other cellular components; (c) filtering the lysate through a filter having a membrane size of equal to or less than 0.4 micron to obtain a filtrate; and (d) applying the filtrate to a culture of aged adult stem cells (AASC) for a time period ranging from about 24 hours to about 30 days to generate a reprogrammed AASC (R-AASC) wherein the AASC were obtained from a second subject and wherein the R-AASC when infused into a breast cancer stem model results in a reduced number of breast cancer cells in bone marrow in comparison to a breast cancer stem model having AASC that have not been reprogrammed. |
| 申请公布号 |
US2016108370(A1) |
申请公布日期 |
2016.04.21 |
| 申请号 |
US201514922353 |
申请日期 |
2015.10.26 |
| 申请人 |
ADVANCED REGEN MEDICAL TECHNOLOGIES, LLC |
发明人 |
Greco Steven J.;Giampapa Vincent C. |
| 分类号 |
C12N5/074;A61K35/12 |
主分类号 |
C12N5/074 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method comprising;
(a) obtaining young adult stem cells (YASC) from a first subject; (b) lysing the YASC to generate a lysate comprising an intracellular matrix (ICM) and other cellular components; (c) filtering the lysate through a filter having a membrane size of equal to or less than 0.4 micron to obtain a filtrate; and (d) applying the filtrate to a culture of aged adult stem cells (AASC) for a time period ranging from about 24 hours to about 30 days to generate a reprogrammed AASC (R-AASC) wherein the AASC were obtained from a second subject and wherein the R-AASC when infused into a breast cancer stem model results in a reduced number of breast cancer cells in bone marrow in comparison to a breast cancer stem model having AASC that have not been reprogrammed. |
| 地址 |
Montclair NJ US |
