A KIT FOR DETECTING MICRO-RNA EXTRACTED FROM A SAMPLE OF BODY FLUID AS WELL AND A METHOD FOR THE DETECTION THEREOF

摘要

A kit for detecting a micro-RNA of interest in at least one sample (C) extracted from a body fluid, including: at least one device (2) including a housing casing (2a) in which at least one housing seat (2b) is obtained for said at least one sample (C), and at least one opening (2c) through which said housing seat (2b) is accessible from the outside; at least one container means (3) for said at least one sample (C), said at least one container means (3) being insertable/disconnectable in/from said housing seat (2b) through said at least one opening (2c); at least one optical excitation group (5), housed in said housing casing (2a), designed to emit at least one excitation light radiation (λ,λ1) towards said at least one housing seat (2b); at least one detection group (6), designed to detect at least one emission light radiation (λ2), that can be generated, in use, by said at least one sample (C), said at least one sample (C) being optically excitable by said at least one excitation light radiation (λ, λ1) emitted by said at least one optical excitation group (5), said at least one detection group (6) being designed to supply at least one electric output signal (SO-signal output) correlated with the quantity, in said at least one sample (C), of said micro-RNA of interest; at least one processing unit (7) designed to receive and process said at least one electric signal (SO) and to output an index correlated with the quantity of said micro-RNA of interest in said at least one sample (C); said at least one container means (3) being made of a material permeable to said at least one excitation light radiation (λ, λ1) and to said at least one emission light radiation (λ2); said at least one group (6) for detecting said emission light radiation (λ2) comprises at least one sensor means (6a) of silicon photomultiplier type.

主权项

1. A kit for detecting a micro-RNA of interest in at least one sample extracted from a body fluid, comprising:
at least one device including a housing casing in which at least one housing seat is obtained for said at least one sample, and at least one opening through which said housing seat is accessible from the outside; at least one container means for said at least one sample, said at least one container means being insertable/disconnectable in/from said housing seat through said at least one opening; at least one optical excitation group, housed in said housing casing, designed to emit at least one excitation light radiation towards said at least one housing seat; at least one detection group, designed to detect at least one emission light radiation, that can be generated, in use, by said at least one sample, said at least one sample being optically excitable by said at least one excitation light radiation emitted by said at least one optical excitation group, said at least one detection group being designed to supply at least one electric output signal correlated with the quantity, in said at least one sample, of said micro-RNA of interest; at least one processing unit designed to receive and process said at least one electric signal and to output an index correlated with the quantity of said micro-RNA of interest in said at least one sample; said at least one container means being made of a material permeable to said at least one excitation light radiation and to said at least one emission light radiation wherein said at least one group for detecting said emission light radiation comprises at least one sensor means of silicon photomultiplier type.