Rapid Nucleic Acid Extraction Method and Apparatus

摘要

A syringe device having a plunger beneath which is disposed a rupturable capsule containing a nucleic acid wash buffer and a rupturing mechanism for rupturing the capsule, wherein the capsule, syringe, plunger, and rupturing mechanism are so configured as to first apply pneumatic pressure to force a nucleic acid solution through a membrane and to thereafter rupture the capsule and force a first volume of the wash buffer through the membrane.

主权项

1. A nucleic acid extraction apparatus comprising:
a container containing a solution comprising nucleic acid, the container further comprising a membrane configured to capture nucleic acid; a rupturable capsule containing a wash buffer; a rupturing element for rupturing said capsule so as to release said wash buffer; a syringe device, said capsule being positioned within said syringe device, above a lower end of said syringe device and beneath a plunger of said syringe device, the lower end of said syringe device being shaped to fit into an open end of said container; the plunger of said syringe device being depressible so as to first force said solution through said membrane such that the membrane retains nucleic acid while passing undesired components of the solution therethrough; and wherein said plunger is further depressible so as to cause said rupturing element to rupture said capsule and release said wash buffer and to thereafter drive said wash buffer out of the lower end of said syringe device and through said membrane, thereby leaving pure nucleic acid on said membrane.