IMPROVED HYBRID SEED PRODUCTION METHOD

摘要

Methods are provided for hybrid seed production using 3-phyletic crosses between female, maintainer and male, particularly restorer lines, wherein the trait is introduced in the female line only at the stage of basic seed production via crossing of the female line with a maintainer line containing the gene or genes encoding the trait in homozygous state.

主权项

1) A method for producing herbicide-tolerant hybrid seed using
a) a male-sterile plant line or A-line; b) a male-sterile plant line comprising a herbicide tolerance gene in heterozygous or hemizygous state or AHT-line; c) an isogenic maintainer plant line or B-line; d) an isogenic maintainer plant line comprising a herbicide tolerance gene in homozygous state or BHT-line; e) and a male fertile line which can be a restorer line or R-line comprising said herbicide tolerance gene in homozygous state; wherein said hybrid seed is produced by crossing plants grown from basic seed of the AHT-line and plants of the male fertile line or R-line and collecting the seeds produced on plants of the A-line, and wherein said basic seed of the AHT-line has been produced by crossing plants grown from pre-basic seed of the A-line with plants of the BHT-line and collecting the seeds produced on plants of the A-line, and wherein said pre-basic seed of the A-line has been produced by crossing plants grown from pre-basic seed of the A-line with plants of the B-line and collecting seeds produced on plants of the A-line.