发明名称 |
REPEATED FED-BATCH CULTURE METHODS |
摘要 |
Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume. |
申请公布号 |
WO2016059541(A1) |
申请公布日期 |
2016.04.21 |
申请号 |
WO2015IB57808 |
申请日期 |
2015.10.12 |
申请人 |
MARA RENEWABLES CORPORATION |
发明人 |
BERRYMAN, KEVIN;SUN, ZHIYONG;MILWAY, MICHAEL;VALENTINE, MERCIA;ARMENTA, ROBERTO E. |
分类号 |
C12Q3/00;C11B1/00;C12M1/36;C12N1/00;C12N1/10;C12N1/14;C12P1/00;C12P7/64 |
主分类号 |
C12Q3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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