| 摘要 |
FIELD: chemistry.SUBSTANCE: present invention relates to analytical bioorganic chemistry. Disclosed fluorescence-labelled dNTP and rybonucleozidtriphosfates have general formula H-L-F, where H is modified at final phosphorus atom natural dNTP or rybonucleozidtriphosfate, L is linker group, connected to final phosphorus atom and constructed on basis of secondary diamines, F is fluorescent reporter group, connected to linker by means of secondary amine groups. Method for synthesis of fluorescent-labelled nucleotides includes connection of the linker to fluorofor, activation triphosfate groups by converting them in cyclic anhydride shape, interaction of obtaining activated with dyes containing linker group, and purification of desired compounds.EFFECT: disclosed fluorescence-labelled nucleotides can be used in methods of single molecular sequencing, expanding range of possible conditions of its performance.4 cl, 4 dwg, 1 tbl, 8 ex |
