Optomechanical module for converting a microscope to provide selective plane illumination microscopy

摘要

A module for engagement with a conventional microscope to provide selective plane illumination microscopy is disclosed. The module is coupled to the translational base of the microscope and defines a mounting having a mount body in which an excitation objective having a first longitudinal axis is engaged to one portion of the mount body and a detection objective having a second longitudinal axis is engaged to another portion of the mount body such that the first longitudinal axis is in perpendicular geometric relation with the second longitudinal axis.

主权项

1. A module for converting a microscope to provide selective plane illumination microcopy comprising:
a mount defining a mount body; an excitation objective having a first longitudinal axis engaged to one portion of the mount body and a detection objective having a second longitudinal axis engaged to another portion of the mount body, wherein the excitation objective and the detection objective are engaged to the mount such that the first longitudinal axis intersects the second longitudinal axis in a perpendicular geometric relation, and a light source for generating in alternating fashion a first plurality of light sheets through the excitation objective and then a second plurality of light sheets through the detection objective, and vice versa, for producing an excitation in a sample that generates an alternating sequence of a first fluorescent emission detected by the excitation objective and then a second fluorescent emission detected by the detection objective, and vice versa.