发明名称 |
Massively parallel combinatorial genetics |
摘要 |
The invention relates to methods and compositions that enable the rapid generation of high-order combinations of genetic elements, and that provide a barcoded basis for rapid characterization of the specific combination of genetic elements encoded within a single cell or in a pooled population. |
申请公布号 |
US9315806(B2) |
申请公布日期 |
2016.04.19 |
申请号 |
US201314411628 |
申请日期 |
2013.06.28 |
申请人 |
Massachusetts Institute of Technology |
发明人 |
Lu Timothy Kuan-Ta;Cheng Allen |
分类号 |
C12N15/70;C12N15/10;C12Q1/68;G01N35/00 |
主分类号 |
C12N15/70 |
代理机构 |
Wolf, Greenfield & Sacks, P.C. |
代理人 |
Wolf, Greenfield & Sacks, P.C. |
主权项 |
1. A genetic construct comprising:
a DNA element; a first compatible end element and a second compatible end element flanking the DNA element, wherein the first and second compatible end elements are capable of annealing to each other; a barcode element; a third compatible end element and a fourth compatible end element flanking the barcode element, wherein the third and fourth compatible end elements are capable of annealing to each other but are not capable of annealing to the first or second compatible end elements; and a separation site located between the fourth compatible end element and the first compatible end element, wherein the DNA element, first compatible end element and second compatible end element are on one side of the separation site, and the barcode element, third compatible end element and fourth compatible end element are on the other side of the separation site. |
地址 |
Cambridge MA US |