Biological microfluidics chip and related methods

摘要

A biological microfluidics chip comprising a substrate, a microfluidic inlet port defining an opening in a surface of the substrate, and a microfluidic outlet port defining an opening in a surface of the substrate. The biological microfluidics chip also comprises a plurality of wells extending from a top surface of the substrate, wherein each well is bounded by one or more walls, and an inlet opening and an outlet opening are provided in a wall of each of the plurality of wells. An inlet microfluidic channel is provided in the substrate to connect the microfluidic inlet port to each of the inlet openings in the walls of the wells, and an outlet microfluidic channel is provided in the substrate to connect each of the outlet openings in the walls of the wells to the microfluidic outlet port.

主权项

1. A biological microfluidics chip comprising:
a substrate; a microfluidic inlet port defining an opening in a surface of the substrate; a microfluidic outlet port defining an opening in a surface of the substrate; a plurality of wells extending from a top surface of the substrate, wherein each well is bounded by one or more walls, and an inlet opening and an outlet opening are provided in a wall of each of the plurality of wells; one or more microfluidic inlet channels in the substrate that connect the microfluidic inlet port to each of the inlet openings in the walls of the wells, wherein the length of the channel between the main artery of the microfluidic inlet channel and the inlet opening in the wall of a well varies such that the entire length of a microfluidic channel between the microfluidic inlet port and an inlet opening in the wall of a well is substantially the same for every well of the plurality of wells within the substrate; one or more microfluidic inlet channels in the substrate that connect each of the outlet openings in the walls of the wells to the microfluidic outlet port; a temperature control inlet port and a temperature control outlet port; and a temperature control channel configured to transport temperature control fluid from the temperature control inlet port to the temperature control outlet port along a path that is in proximity to one or more of the plurality of wells such that, in use, heat is exchangeable between the temperature control liquid and the contents of the wells.