Method and apparatus for target detection using electrode-bound viruses

摘要

A biosensor that is capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ΔZRe of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate.

主权项

1. A biosensor comprising:
an electrically conductive gold electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ΔZRe of the electrode; a self-assembled monolayer chemically bonded to a surface of the electrically conductive gold electrode via a thiol-gold bond formed between N-hydroxysuccinimide thioctic ester and the gold electrode; and a plurality of phage virus particles covalently bound to the self-assembled monolayer.