Reprogramming cells

摘要

The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.

主权项

1. A method of inducing a non-pluripotent mammalian cell into an induced pluripotent stem cell, the method comprising:
(a) introducing into the non-pluripotent cell a polynucleotide encoding an Oct4 polypeptide; and (b) contacting the non-pluripotent cell with a small molecule 3′-phosphoinositide-dependent kinase-1 (PDK1) activator, a TGFβ receptor/ALK5 inhibitor, and a MEK inhibitor, thereby inducing the non-pluripotent mammalian cell into an induced pluripotent stem cell.